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1974
Huez, G, Marbaix G, Hubert E, Leclercq M, Nudel U, Soreq H, Salomon R, Lebleu B, Revel M, Littauer UZ.  1974.  Proceedings: Role of the polyadenylic segment in the translation of globin messenger RNA. Hoppe Seylers Z Physiol Chem. 355:1211.
Minke, B, Hochstein S, Hillman P.  1974.  Derivation of a quantitative kinetic model for a visual pigment from observations of early receptor potential. Biophysical Journal. 14:490–512. Abstract
A "complete" and quantitative kinetic model for the states and transitions of the barnacle visual pigment in situ has been constructed from intracellular recordings of the early receptor potential responses to long light pulses. The model involves two stable and four thermolabile states and 10 photochemical, thermal, and metabolic transitions among them. The existence of each state and transition is demonstrated by qualitative examination of the response resulting from a carefully chosen experimental paradigm (combination of intensity, duration, and wavelength of adaptation and stimulation). Quantitative examination of the same responses determines all of the model transition rates, but only puts constraints on the state dipole moments. The latter are determined, and the former refined, by quantitative comparison of the predictions of the complete model with the responses to a set of paradigms chosen to involve as many states and transitions as possible. The fact that good fits can be obtained to these responses without further modification of the model supports its completeness.
Huez, G, Marbaix G, Hubert E, Leclercq M, Nudel U, Soreq H, Salomon R, Lebleu B, Revel M, Littauer UZ.  1974.  Role of the polyadenylate segment in the translation of globin messenger RNA in Xenopus oocytes. Proc Natl Acad Sci U S A. 71:3143-6. Abstract
The translations of native messenger RNA for rabbit globin and that of poly(A)-free globin messenger RNA have been compared after injection into Xenopus oocytes. The initial rate of translation of poly(A)-free mRNA is close to that found with intact mRNA. However, at longer incubation periods, the rate of globin synthesis with poly(A)-free mRNA is considerably lower than with native mRNA. Similar differences in the template activity of the two mRNA preparations were found with a cell-free extract of Krebs II ascites tumor. It is concluded that the presence of the 3' poly(A)-rich sequence in mRNA is required to ensure high functional stability.
Minke, B, Hochstein S, Hillman P.  1974.  Derivation of a quantitative kinetic model for a visual pigment from observations of early receptor potential. 14(6):490-512.
1973
Hochstein, S, Minke B, Hillman P.  1973.  Antagonistic components of the late receptor potential in the barnacle photoreceptor arising from different stages of the pigment process. The Journal of General Physiology. 62:105–128. Abstract
The late receptor potential {(LRP)} recorded in barnacle photoreceptor cells exhibits, at high light levels, a strong dependence on the color of the stimulus and of the preceding adaptation. Most strikingly, red illumination of a cell previously adapted to blue light results in a depolarization which may last for up to 30 min after the light goes off, while blue illumination of a cell previously adapted to red light cuts short this extended depolarization or prevents its induction by a closely following red light. Comparison of the action spectra for the stimulus-coincident {LRP} and for the extended depolarization and its curtailment with those previously measured for the early receptor potential {(ERP)} confirms that these phenomena derive from the same bi-stable pigment as the {ERP.} The stimulus-coincident response and the extended depolarization appear to arise from substantial activation of the stable 532 nm state of the pigment, while activation of the stable 495 state depresses or prevents the extended depolarization and probably also depresses the stimulus-coincident response. Since either process can precede the other, with mutually antagonistic effects, one is not simply the reversal of the other; they must be based on separate mechanisms. Furthermore, comparison with {ERP} kinetics shows that both processes involve mechanisms additional to the pigment changes, as seen in the {ERP.} A model is proposed and discussed for the {LRP} phenomena and their dependences on wavelength, intensity, and duration of illumination based on excitor-inhibitor interactions.
Minke, B, Hochstein S, Hillman P.  1973.  Early receptor potential evidence for the existence of two thermally stable states in the barnacle visual pigment. The Journal of General Physiology. 62:87–104. Abstract
The early receptor potential {(ERP)} in the barnacle photoreceptor is shown by intracellular recording to exhibit a strong dependence on the color of the stimulus and of the preceding adaptation. The adaptation effects appear to be stable for at least 3 h in the dark. Most strikingly, the {ERP} is positive after red adaptation and mainly negative after blue adaptation. The simplest hypothesis which accounts for these observations is that two thermally stable pigment states with different absorption spectra contribute to the {ERP.} All {ERP} responses appear to be consistent with the sums of different ratios of the {ERP's} of the two pure states. The relative populations of the two states are shown to vary reciprocally, suggesting that the two are states of the same closed pigment cycle. Both states have approximately Dartnall nomogram-shaped absorption spectra, one peaked near 495 nm, and the other near 532 nm.
Hillman, P, Dodge FA, Hochstein S, Knight BW, Minke B.  1973.  Rapid dark recovery of the invertebrate early receptor potential. The Journal of General Physiology. 62:77–86. Abstract
The recovery in the dark of the early receptor potential, as a direct manifestation of the state of the visual pigments, has been studied by intracellular recording in the ventral photoreceptors of Limulus and lateral photoreceptors of Balanus. The recovery is exponential with 1/e time constants of about 80 ms at 24 degrees C for both preparations and 1800 ms at 4 degrees C for Balanus. The 24 degrees C rate extrapolates to total recovery of the pigment within 2 s. The later part of the dark adaptation of the late receptor potential, which may take from seconds to minutes in these preparations, appears thus to be unrelated to the state of the pigment.
Minke, B, Hochstein S, Hillman P.  1973.  Letter: Antagonistic process as source of visible-light suppression of afterpotential in Limulus {UV} photoreceptors. The Journal of General Physiology. 62:787–791.
Hochstein, S, Minke B, Hillman P.  1973.  Antagonistic components of the late receptor potential in the barnacle photoreceptor arising from different stages of the pigment process. 62(1):105-128.
Minke, B, Hochstein S, Hillman P.  1973.  Early receptor potential evidence for the existence of two thermally stable states in the barnacle visual pigment. 62(1):87-104.
Minke, B, Hochstein S, Hillman P.  1973.  Letter: Antagonistic process as source of visible-light suppression of afterpotential in Limulus UV photoreceptors. 62(6):787-791.
Hillman, P, Dodge FA, Hochstein S, Knight BW, Minke B.  1973.  Rapid dark recovery of the invertebrate early receptor potential. 62(1):77-86.
Minke, B, Hochstein S, Hillman P.  1973.  Photoreceptor transduction. A new system. Israel Journal of Medical Sciences. 9 Suppl:114–118.
1972
Hochstein, S, Minke B, Hillman P.  1972.  Receptor potentials from a visual pigment with two thermally stable states. 24:65-73.
Hillman, P, Hochstein S, Minke B.  1972.  A visual pigment with two physiologically active stable states. Science. 175(29):1486-1488.
Hochstein, S, Minke B, Hillman P.  1972.  Receptor potentials from a visual pigment with two thermally stable states. Advances in Experimental Medicine and Biology. 24:65–73.
Hillman, P, Hochstein S, Minke B.  1972.  A visual pigment with two physiologically active stable states. Science {(New} York, {N.Y.)}. 175:1486–1488. Abstract
Red illumination of a Balanus amphitrite photoreceptor that has been adapted to blue light leads to prolonged depolarization in the late receptor potential. This depolarization can be switched off by further exposure to a blue stimulus. The early receptor potential in this cell is purely depolarizing or largely hyperpolarizing; the former is true if the cell has been adapted to red light, and the latter, if blue light has been used. The color-adaptation "memories" for both early and late receptor potentials appear to be permanent. The existence of two stable states for the early receptor potential directly implies a pigment with two stable states, and these apparently contribute antagonistically to the late receptor potential.
1971
Hillman, P, Hochstein S, Minka B.  1971.  Receptor potentials from a pigment system with two stable states. Vision Research. 11:1193.
Soreq, H, Kaplan R.  1971.  Inducible and constitutive -galactosidase formation in cells recovering from protein synthesis inhibition. J Bacteriol. 108:1147-53. Abstract
Inducible and constitutive beta-galactosidase formation and radioactive amino acid incorporation were measured in cells recovering from various treatments which inhibit protein synthesis in the cell. Undelayed beta-galactosidase formation was found in stringent auxotrophs recovering from amino acid starvation, in cells recovering from glycerol or potassium starvation, and in bacteria recovering from puromycin treatment. Delayed beta-galactosidase formation was found in relaxed auxotrophs recovering from amino acid starvation and in prototrophs recovering from chloramphenicol or from tetracycline treatment. The length of this delay was directly proportional to the duration of the treatment. All cells recovering from the various treatments exhibited a slightly decreased rate of beta-galactosidase formation and an increase in radioactive amino acid incorporation.