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Home » Publications » Neuronal-expressed microRNA-targeted pseudogenes compete with coding genes in the human brain

Neuronal-expressed microRNA-targeted pseudogenes compete with coding genes in the human brain

MicroRNAs orchestrate brain functioning via interaction with microRNA recognition elements (MRE) on target transcripts. However, the global impact of potential competition on the microRNA pool between coding and non-coding brain transcripts that share MREs with them remains unexplored. Here we report that non-coding pseudogene transcripts carrying MREs (PSG+MRE) often show duplicated origin, evolutionary conservation and higher expression in human temporal lobe neurons than comparable duplicated MRE-deficient pseudogenes (PSG-MRE). PSG+MRE participate in neuronal RNA-induced silencing complexes (RISC), indicating functional involvement. Furthermore, downregulation cell culture experiments validated bidirectional co-regulation of PSG+MRE with MRE-sharing coding transcripts, frequently not their mother genes, and with targeted microRNAs; also, PSG+MRE single-nucleotide polymorphisms associated with schizophrenia, bipolar disorder and autism, suggesting interaction with mental diseases. Our findings indicate functional roles of duplicated PSG+MRE in brain development and cognition, supporting physiological impact of the reciprocal co-regulation of PSG+MRE with MRE-sharing coding transcripts in human brain neurons.

Authors: Barbash S, Simchovitz A, Buchman AS, Bennett DA, Shifman S, Soreq H.

Year of publication: 2017

Journal: Transl Psychiatry. 2017 Aug 8;7(8):e1199

Link to publication:

https://doi.org/10.1038/tp.2017.163

Labs:

Hermona Soreq

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