Megakaryocytopoiesis was selectively inhibited in cultured murine bone marrow cells by a 15-mer oligodeoxynucleotide complementary to the initiator AUG region in butyrylcholinesterase mRNA. Furthermore, conditioned medium from Xenopus oocytes producing recombinant butyrylcholinesterase stimulated megakaryocytopoiesis. These observations implicate butyrylcholinesterase in megakaryocytopoiesis and suggest application of oligodeoxynucleotides for modulating bone marrow development.
Manipulations of cholinesterase gene expression modulate murine megakaryocytopoiesis in vitro
Authors: D Patinkin, S Seidman, F Eckstein, F Benseler, H Zakut, and H Soreq
Year of publication: 1990
Journal: Mol Cell Biol. 1990 Nov; 10(11): 6046–6050.
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