Baruch Minke Lab

ELSC Members

Research Group of TRP Channels in Vision and Pain

My investigations have created a new field of research. I co-discovered a new type of ion channel, which I designated the Transient Receptor Potential or in short the TRP channel, as a result of my studies on phototransduction and vision in fruit flies (Drosophila melanogaster). I investigated the biophysical and biochemical properties of TRP channels in fruit fly eyes and identified together with the late Prof. Zvi Selinger phospholipase C as a crucial component of the Drosophila TRP signaling pathway. The principles that we found are common to numerous sensory (e.g. temperature, pain and taste sensations) and motor systems (properties of Purkinje neurons in the cerebellum), thus laying the foundation for the study of molecules that underlie mechanisms of TRP and pain, originally initiated by Prof. David Julius. Today we have extended our research to the first identified loss of function mutation in human TRPV1 channel that govern at least several of the human body’s regulatory processes related inflammatory pain and to noxious heat detection. Our major aim is to decipher the critical roles of TRP channels in invertebrate vision and of TRPV1 in human somatosensory physiology.

Envelope Phone-alt

Baruch Minke

Professor Emeritus

Email: baruchm@ekmd.huji.ac.il
Phone: +972-2-6758407
Fax: +972-2-6439736
Mobile: +972-54-7961151
Baruch Minke CV
Address: Department of Medical Neurobiology, Faculty of Medicine,
Hadassah Ein-Kerem Campus, The Hebrew University of Jerusalem
Research Group of TRP Channels in Vision and Pain

My investigations have created a new field of research. I co-discovered a new type of ion channel, which I designated the Transient Receptor Potential or in short the TRP channel, as a result of my studies on phototransduction and vision in fruit flies (Drosophila melanogaster). I investigated the biophysical and biochemical properties of TRP channels in fruit fly eyes and identified together with the late Prof. Zvi Selinger phospholipase C as a crucial component of the Drosophila TRP signaling pathway. The principles that we found are common to numerous sensory (e.g. temperature, pain and taste sensations) and motor systems (properties of Purkinje neurons in the cerebellum), thus laying the foundation for the study of molecules that underlie mechanisms of TRP and pain, originally initiated by Prof. David Julius. Today we have extended our research to the first identified loss of function mutation in human TRPV1 channel that govern at least several of the human body’s regulatory processes related inflammatory pain and to noxious heat detection. Our major aim is to decipher the critical roles of TRP channels in invertebrate vision and of TRPV1 in human somatosensory physiology.

Ben Katz
Ben Katz PhD
Research Associate
ELSC-Seminars-General
Elisheva Rhodes-Mordov
PhD Student
ELSC-Seminars-General
Rachel Zaguri
PhD Student
ELSC-Seminars-General
Rita Gutotov
PhD Student
ELSC-Seminars-General
Tal Brandwine
PhD Student
Brandwine Tal, Ifrah Reut, Bialistoky Tzofia, Zaguri Rachel, Rhodes-Mordov Elisheva, Mizrahi-Meissonnier Liliana, Sharon Dror, Katanaev Vladimir L., Gerlitz Offer, Minke Baruch

Knockdown of Dehydrodolichyl Diphosphate Synthase in the Drosophila Retina Leads to a Unique Pattern of Retinal Degeneration

Frontiers in Molecular Neuroscience (2021)

Barbera NA, Minke B, Levitan I

Comparative docking analysis of cholesterol analogs to ion channels to discriminate between stereospecific binding vs. stereospecific response

Channels, Volume 13, Issue 1, Pages 136-146 (2019)

Gutorov R, Peters M, Katz B, Brandwine T, Barbera NA, Levitan I, Minke B

Modulation of Transient Receptor Potential C Channel Activity by Cholesterol

Frontiers in Pharmacology 10, 1487 (2019)

Katz, B., Pak, W. L., and Minke, B.

TRPC Channels—Insight from the Drosophila Light Sensitive Channels

Oxford University New York, NY 10016, Oxford Handbooks Online (In Press) (2019)

Katz B, Minke B.

The Drosophila light-activated TRP and TRPL channels – Targets of the phosphoinositide signaling cascade.

Progress in Retinal and Eye Research, Volume 66, Pages 200-219 (2018)

Peters M, Katz B, Lev S, Zaguri R, Gutorov R, Minke B.

Depletion of Membrane Cholesterol Suppresses Drosophila Transient Receptor Potential-Like (TRPL) Channel Activity.

Current Topics in Membranes, 80:233-254. (2017)

Yasin B, Kohn E, Peters M, Zaguri R, Weiss S, Schopf K, Katz B, Huber A, Minke B.

Ectopic Expression of Mouse Melanopsin in Drosophila Photoreceptors Reveals Fast Response Kinetics and Persistent Dark Excitation.

Journal of Biological Chemistry, 292(9):3624-3636. (2017)

Katz B*, Gutorov R*, Rhodes-Mordov E, Hardie RC, Minke B.

Electrophysiological Method for Whole-cell Voltage Clamp Recordings from Drosophila Photoreceptors.

Journal of Visualized Experiments, (124). (2017)

Katz B, Voolstra O, Tzadok H, Yasin B, Rhodes-Modrov E, Bartels JP, Strauch L, Huber A, Minke B.

The latency of the light response is modulated by the phosphorylation state of Drosophila TRP at a specific site.

Channels (Austin), 11(6):678-685. (2017)

Voolstra O, Rhodes-Mordov E, Katz B, Bartels JP, Oberegelsbacher C, Schotthöfer SK, Yasin B, Tzadok H, Huber A, Minke B.

The Phosphorylation State of the Drosophila TRP Channel Modulates the Frequency Response to Oscillating Light In Vivo.

Journal of Neuroscience, 37 (15) 4213-4224 (2017)

Katz, B., Payne, R., and Minke, B.

TRP channels in vision.

Neurobiology of TRP Channels, T. Rosenbaum, Editor, CRC Press/Taylor and Francis Group (2017)

Weiss, S. and Minke B.

A new genetic model for calcium induced autophagy and ER-stress in Drosophila photoreceptor cells

Channels, Volume 9, Issue 1, pages 14-20 (2015)

Kohn E, Katz B, Yasin B, Peters M, Rhodes E, Zaguri R, Weiss S, Minke B.

Functional cooperation between the IP3 receptor and phospholipase C secures the high sensitivity to light of Drosophila photoreceptors in vivo

J Neurosci. 2015 Feb 11;35(6):2530-46 (2015)

Katz B, Oberacker T, Richter D, Tzadok H, Peters M, Minke B, Huber A.

Drosophila TRP and TRPL are assembled as homomultimeric channels in vivo

J Cell Sci. 2013 Jul 15;126(Pt 14):3121-33 (2013)

Peters M., Trembovler V., Alexandrovich A., Parnas M., Birnbaumer L., Minke B., Shohami E

Carvacrol Together with TRPC1 Elimination Improve Functional Recovery after Traumatic Brain Injury in Mice

Journal of Neurotrauma, Vol. 29, No. 18 (2012)

Weiss S, Kohn E, Dadon D, Katz B, Peters M, Lebendiker M, Kosloff M, Colley NJ, Minke B.

Compartmentalization and Ca2+ Buffering Are Essential for Prevention of Light-Induced Retinal Degeneration

J Neurosci. 2012 Oct 17;32(42):14696-708 (2012)

Katz B. and Minke B

Phospholipase C-Mediated Suppression of Dark Noise Enables Single-Photon Detection in Drosophila Photoreceptors

Journal of Neuroscience, 32 (8) 2722-2733 (2012)

Lev S., Katz B, Tzarfaty V. and Minke B

Signal-dependent hydrolysis of phosphatidylinositol 4,5-bisphosphate without activation of phospholipase C: implications on gating of Drosophila TRPL (transient receptor potential-like) channel

The Journal of Biological Chemistry, 287, 1436-1447 (2012)

Lev S, Katz B, Minke B

The activity of the TRP-like channel depends on its expression system

Channels, Volume 6, Issue 2 (2012)

Minke B

The History of the Prolonged Depolarizing Afterpotential (PDA) and Its Role in Genetic Dissection of Drosophila Phototransduction

Journal of Neurogenetics, Volume 26, Issue 2 (2012)

Minke B, Peters M

Rhodopsin as Thermosensor?

Science, Vol. 331, Issue 6022, pp. 1272-1273 (2011)

Richter D., Katz B., Oberacker T., Tzarfaty V., Belusic G., Minke B., Huber A

Translocation of the Drosophila Transient Receptor Potential-like (TRPL) Channel Requires Both the N- and C-terminal Regions Together with Sustained Ca2+ Entry

The Journal of Biological Chemistry, 286, 34234-34243 (2011)

Dadon D. and Minke B

Cellular functions of Transient Receptor Potential channels

The International Journal of Biochemistry & Cell Biology, Volume 42, Issue 9, Pages 1430-1445 (2010)

Lev S., Zeevi D. A., Frumkin A., Offen-Glasner V., Bach G. and Minke B

Constitutive Activity of the Human TRPML2 Channel Induces Cell Degeneration

The Journal of Biological Chemistry, 285, 2771-2782 (2010)

Lev S. and Minke B

Constitutive Activity of TRP Channels: Methods for Measuring the Activity and Its Outcome

Methods in Enzymology, Volume 484, Pages 591-612 (2010)

Zeevi D. A., Lev S., Frumkin A., Minke B

Heteromultimeric TRPML channel assemblies play a crucial role in the regulation of cell viability models and starvation-induced autophagy

J Cell Sci, 123: 3112-3124 (2010)

Minke B

The History of the Drosophila TRP Channel: The Birth of a New Channel Superfamily

Journal of Neurogenetics, Volume 24, Issue 4 (2010)

Parnas M., Peters M., Dadon D., Lev S., Vertkin I., Slutsky I. and Minke B

Carvacrol is a novel inhibitor of Drosophila TRPL and mammalian TRPM7 channels

Cell Calcium, Volume 45, Issue 3, Pages 300-309 (2009)

Katz B and Minke B

Drosophila photoreceptors and signaling mechanisms

Frontiers in Cellular Neuroscience (2009)

Parnas M, Peters M., Minke B

Linoleic acid inhibits TRP channels with intrinsic voltage sensitivity: Implications on the mechanism of linoleic acid action

Channels (Austin), 3(3):164-6 (2009)

Parnas M, Katz B., Lev S., Tzarfaty V., Dadon D., Gordon-Shaag, A., Metzner H., Yaka R. and Minke B

Membrane Lipid Modulations Remove Divalent Open Channel Block from TRP-Like and NMDA Channels

Journal of Neuroscience, 29 (8) 2371-2383 (2009)

Parnas M., Katz B. and Minke B

Open Channel Block by Ca2+ Underlies the Voltage Dependence of Drosophila TRPL Channel

The Journal of General Physiology, 129 (1) 17-28 (2007)

Frechter S., Elia N., Tzarfaty V., Selinger Z. and Minke B

Translocation of Gqα Mediates Long-Term Adaptation in Drosophila Photoreceptors

Journal of Neuroscience, 27 (21) 5571-5583 (2007)

Minke B. and M. Parnas

Insights on TRP Channels from In Vivo Studies in Drosophila

Annual Review of Physiology, Volume 68, pp 649-684 (2006)

Frechter, S and Minke B

Light-regulated translocation of signaling proteins in Drosophila photoreceptors

Journal of Physiology-Paris Volume 99, Issues 2–3, Pages 133-139 (2006)

Meyer NE, Joel-Almagor T, Frechter S, Minke B, Huber A

Subcellular translocation of the eGFP-tagged TRPL channel in Drosophila photoreceptors requires activation of the phototransduction cascade

Journal of Cell Science, 119: 2592-2603 (2006)

Minke B

TRP channels and Ca2+ signaling

Cell Calcium, Volume 40, Issue 3, Pages 261-275 (2006)

Elia, N., Frechter, S., Minke, B, and Selinger, Z

Excess of Gβe over Gqαe in vivo prevents dark, spontaneous activity of Drosophila photoreceptors

The Journal of Cell Biology, 171 (3) 517-526 (2005)

Chorna-Ornan I. , Tzarfati, V., Ankri-Eliahoo, G., Joel-Almagor, T., Huber A., Payre, F. and B Minke

Light-regulated interaction of Dmoesin with TRP and TRPL channels is required for maintenance of photoreceptors

The Journal of Cell Biology, 171 (1) 143-152 (2005)

Agam, K., Frechter, S. and Minke, B

Activation of the Drosophila TRP and TRPL channels requires both Ca2+ and protein dephosphorylation

Cell Calcium, Volume 35, Issue 2, Pages 87-105 (2004)

Ikhine , R. Corna-Ornan, I. Zars, T. D. Elagina, R. B. Elia, N. Cheng, Y. Selinger, Z. Minke, B. and Hyde, D. R

Novel Dominant Rhodopsin Mutation Triggers Two Mechanisms of Retinal Degeneration and Photoreceptor Desensitization

Journal of Neuroscience, 24 (10) 2516-2526 (2004)

Kosloff, M. Elia, N. Joel-Almagor, T. Timberg, R. Zars T. D. Hyde, D. R. Minke, B. and Selinger, Z

Regulation of light‐dependent Gqα translocation and morphological changes in fly photoreceptors

The EMBO Journal, 22, 459-468 (2003)

Minke, B. and Agam, K

TRP gating is linked to the metabolic state and maintenance of the Drosophila photoreceptor cells

Cell Calcium, Volume 33, Issues 5–6, Pages 395-408 (2003)

Bähner, M., Frechter, S., Da Silva, N., Minke, B., Paulsen, R. and Huber, A

Light-Regulated Subcellular Translocation of Drosophila TRPL Channels Induces Long-Term Adaptation and Modifies the Light-Induced Current

Neuron, Volume 34, Issue 1, Pages 83-93 (2002)

Minke, B. and Cook, B

TRP Channel Proteins and Signal Transduction

Physiol. Rev. 82:429-472 (2002)

Chorna-Ornan, I., Joel-Almagor, T., Cohen Ben-Ami, H., Frechter, S., Gillo, B., Selinger, Z., Gill, D.L. , and Minke, B

A Common Mechanism Underlies Vertebrate Calcium Signaling and Drosophila Phototransduction

Journal of Neuroscience, 21 (8) 2622-2629 (2001)

Minke, B

The TRP Channel and Phospholipase C-Mediated Signaling

Cellular and Molecular Neurobiology, Volume 21, Issue 6, pp 629–643 (2001)

Agam, K., M. von-Campenhausen, S. Levy, H. Cohen Ben-Ami, B. Cook, K. Kirschfeld and B. Minke

Metabolic Stress Reversibly Activates the Drosophila Light-Sensitive Channels TRP and TRPL In Vivo

Journal of Neuroscience, 20 (15) 5748-5755 (2000)

Yoon J, Cohen Ben-Ami H, Hong YS, Park S, Strong LLR, Bowman J, Geng C, Baek K, Minke B, Pak WL

Novel Mechanism of Massive Photoreceptor Degeneration Caused by Mutations in the trp Gene of Drosophila

Journal of Neuroscience, 20 (2) 649-659 (2000)

Cook B, Bar-Yaacov M, Cohen Ben-Ami H, Goldstein R.E., Paroush Z, Selinger Z and Minke B

Phospholipase C and termination of G-protein-mediated signalling in vivo

Nature Cell Biology, volume 2, pages 296–301 (2000)

At this time there are no available positions in the lab.
Honors:
EMET Prize
Prince of Asturias Award (from the current King of Spain)
Envelope Phone-alt

Baruch Minke

Professor Emeritus

Email: baruchm@ekmd.huji.ac.il
Phone: +972-2-6758407
Fax: +972-2-6439736
Mobile: +972-54-7961151
Baruch Minke CV
Address: Department of Medical Neurobiology, Faculty of Medicine,
Hadassah Ein-Kerem Campus, The Hebrew University of Jerusalem

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“Working memory”

New exhibition titled “Working memory” by the artist Alon kedem and in collaboration with ELSC Prof. Leon Deouell. In the picture: Moshe Lion, the mayor of Jerusalem and Prof. Asher Cohen, the president of The Hebrew University of Jerusalem.

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