ELSC Seminar Series

Molecular characterization of brain tissues using optical methods present a problem of scale: brain tissues are intrinsically three-dimensional structures, with thickness of at least hundreds of micrometers; but nanoscale interrogation is needed to characterize molecules within neurites and synapses. Additionally, multiplexed interrogation of molecules is needed to characterize cell types and states inside brain tissues, and to detect deficiencies in neurological conditions. Currently, multiplexed imaging of molecules inside brain tissues is limited to thin sections, and almost impossible with super-resolution. Here we demonstrate multiplexed super-resolved characterization of thick brain tissues: complete fruit fly brain, human brain organoids, and mouse cortex.