Unit Facilities

Levi-Montalcini confocal microscope – Leica Stellaris 5 with a super-continuum white light laser

This microscope is designed for high-resolution 3D imaging of turbid samples

Key features:

  1. Fluorescence excitation at any arbitrary wavelength in the 485-685nm range, besides the conventional 405nm DAPI excitation channel.
  2. Separating spectrally-overlapping fluorophores based on their fluorescence lifetime, and user-selectable collection wavelength ranges.
  3. Built-in algorithms for image deconvolution and automated analysis.
  4. A confocal reflectance mode that can be used for label-free imaging of axonal myelination.
  5. Best in-class user friendliness.
The microscope is equipped with dry 10x and 20x objective lenses, as well as glycerol 20x, 40x and 63x objective lenses. Please read our usage instructions carefully. Click here to watch a training video for this microscope.

Wide-field fluorescence microscope - Olympus IX83P2ZF

This microscope is designed for fast, sensitive imaging of large sections.

Key features:

    • 9-slide stage
    • Plate stage
    • Universal dish stage (35mm and 60mm)
    • 95% Quantum efficiency camera: 2048×2048 pixels with 16-bit depth
    • 6 separate illumination LEDs, 7 excitation filters, 6 dichroic mirrors and 7 emission filters for every practical combination of excitation and emission.
    • Fast filter wheels
    • A wide variety of objective lenses:
      • 4x
      • 10x
      • 20x – long working distance
      • 20x – high NA
      • 40x
      • 60x water
    • Hardware-based, fast, continuous autofocus (not an option for 4x magnification).
    • Quad dichroic mirror for high-speed channel switching (DAPI, GFP, mCherry, Cy5) and individual mirrors with high sensitivity (for CFP, YFP, GFP, mCherry, Cy5).

LED excitation lights – Lumencor spectraX

    • Violet: 395/25nm, 295mW
    • Blue: 440/20nm, 256mW
    • Cyan: 470/24nm, 196mW
    • Teal: 510/62nm, 62mW
    • Green-Yellow: 570/80nm, ~600mW
    • Red: 640/30nm, 231mW

Excitation filters (on fast wheel):

  • ET395/25x
  • ET470/40x
  • ET485/25x
  • ET550/20x
  • ET560/40x
  • ET620/60x
  • ET640/30x
  • Free

ET436/20x – permanent on blue LED

Dichroic mirrors (separate wheel):

    • 89100bs – sedat quad for fast switching
    • T455lp
    • T495lpxr
    • T565lpxr
    • T585lpxr
    • T660lpxr
    • Open slot for custom filter cubes
    • Free

Emission filters (fast wheel):

    • ET455/50 nm
    • ET480/40 nm
    • ET525/36 nm
    • ET525/50 nm
    • ET605/52 nm
    • ET630/75 nm
    • ET705/72 nm
    • Free

To identify the right filter/fluorophore combination for your experiment, please use: https://www.fpbase.org/spectra/ This tool supports all catalog numbers for components and all possible fluorophores.

Multi-photon microscopes - LOTOS

These two microscopes are designed for deep imaging through light-scattering tissues, such as the living brain.

Key features:

  • Two independent microscopes (Cajal & Golgi), both fed by a Chameleon Vision II femtosecond laser.
  • Acquisition rates of up to 1,000 frames per second using resonant-galvo scanners, implementing Low-power Temporal Over-Sampling (LOTOS) imaging for reduced photodamage.
  • PMT2101/M GaAsP PMTs.
  • Rapid beam power modulation using Conoptics 350-105 / 350-50 electro-optic modulators and 302RM high voltage amplifiers.
  • Light-tight, soundproof Faraday cages.
  • Ultra-stable beam alignment using 3D Optix optomechanics.


Future upgrades:

  • Time-tagged photon counting with a temporal precision of 42 picoseconds, for improved signal-to-background and ΔF/F ratios.
  • Fluorescence lifetime microscopy, second harmonic generation (SHG) and gated imaging.
  • Rapid volumetric imagery of the living brain at up to 320 volumes per second. (Pending user demand)
  • Super-continuum femtosecond excitation for deeper, non-degenerate two-photon microscopy at ~1,300nm and ~1,650nm. (Pending user demand)

Light Sheet Microscope - La-Vision Ultramicroscope II

This microscope is designed for fast, high-resolution scans of large cleared samples (e.g., with iDISCO or Clarity).

Key features:

    • A chemical hood for safe work with organic solvents (e.g., DBE and ECi).
    • 3 sheets for minimal light obstruction.
    • A huge working distance (> 9mm) for most objective lenses
    • High-end sCMOS camera: 2,560×2,160 pixels, pixel size 6.5×6.5 μm²
    • Dynamic focus for axial sweeping.
    • Fixed lens configuration:
      • 3x/2.6x
      • 4x/8x
      • 12x/24x
    • Zoom body configuration
      • 63-6.3x adjustable zoom

Excitation lasers:

  1. 405nm: 100mW
  2. 488nm: 85mW
  3. 561nm: 100mW
  4. 639nm: 70mW
  5. 785nm: 75mW

Emission filters:

  1. 460/40 nm
  2. 525/50 nm
  3. 620/60 nm
  4. 680/30 nm

HIVE analysis server

The HIVE is an analysis and communication server designed for imaging core facilities. It is composed of three servers:

  • A network server, which is connected directly by 10 Gbit wiring to each microscope and allows data transfer rates of up to 1 Gbit per second, facilitating online analysis and direct acquisition to the HIVE storage. In addition, the server provides fast remote access to the HIVE with a personalized Windows environment.
  • A storage NAS RAID6 server, which supplies 52TB (easily expandable) of super high-speed (up to 2.2 Gbit per second) safe storage for analysis.
  • A core Windows 2019 processing server, which holds high-speed 512 GB RAM (expandable to 2 TB) with Intel Xeon Silver 20 Core/40 thread CPUs and NVIDIA RTX8000 with 48 GB GPU-RAM (biggest capacity GPU on the market).

For more information, see: https://www.acquifer.de/data-solutions/

“Working memory”