Structured illumination microscopies achieve optical sectioning via differential modulation of in-focus and out-of-focus contributions to an image. Multiple wide-field camera images are analyzed to recreate an optical section. The requirement for multiple camera frames per image entails a loss of temporal resolution compared to conventional wide-field imaging. Here we describe a computational structured illumination imaging scheme, compressed Hadamard imaging, which achieves simultaneously high spatial and temporal resolution for optical sectioning of 3D samples with low-rank dynamics (e.g. neurons labeled with fluorescent activity reporters). We validate the technique with numerical simulations, and then illustrate with wide-area optically sectioned recordings of membrane voltage dynamics in mouse neurons in an acute brain slice and of calcium dynamics in zebrafish brain in vivo.
Year of publication
Journal of Physics D: Applied Physics, Volume 52, Number 14